C3 And C4 Pathways - Steps, Differences And FAQ

C3 And C4 Pathways - Steps, Differences And FAQ

Irshad AnwarUpdated on 29 Oct 2025, 11:58 AM IST

C3 and C4 plants differ in their photosynthetic pathways, environmental adaptations, and photorespiration rates. C3 plants follow the Calvin cycle, while C4 plants use the Hatch–Slack pathway with Kranz anatomy, allowing them to thrive in hot and dry conditions.

This Story also Contains

  1. What Is Photosynthesis?
  2. What Are C3 and C4 Plants?
  3. Major Differences Between C3 and C4 Plants
  4. Kranz Anatomy in C4 Plants
  5. Adaptive and Ecological Significance
  6. C3 vs C4 Pathway NEET MCQs (With Answers & Explanations)
C3 And C4 Pathways - Steps, Differences And FAQ
C3 And C4 Pathways

What Is Photosynthesis?

Photosynthesis is the process through which plants convert light energy to chemical energy in the form of glucose. This can happen through different pathways, mainly the C3 and C4 pathways. Knowing what makes the two different plays a key role in the NEET exam, as these adaptations by plants to different environmental conditions are brought out. difference between C3 and C4 Plants

C3 and C4 plants are fundamentally very different in photosynthetic pathways, adaptations, and environmental preferences. Differences between C3 and C4 Plants are explained herein in detail.

What Are C3 and C4 Plants?

The C3 and C4 plants are described below:

C3 Plants

  • These plants perform photosynthesis via the Calvin cycle/

  • The first stable product is 3-phosphoglycerate (3-PGA), a 3-carbon compound.

  • Occur mainly in cool, moist environments.

  • Example: Wheat, rice, potato, soybean, barley

C4 Plants

  • These plants follow the Hatch-Slack pathway (C4 cycle) before the Calvin cycle.

  • The first stable product is oxaloacetic acid (OAA), a 4-carbon compound.

  • Occur in hot, dry environments.

  • Example: Maize, sugarcane

Major Differences Between C3 and C4 Plants

The major difference between C3 and C4 plants include:

Feature

C3 Plants

C4 Plants

Photosynthesis Pathway

C3 pathway (Calvin cycle)

C4 pathway (Hatch-Slack pathway)

First Stable Product

3-phosphoglycerate (3-PGA)

Oxaloacetate (4-carbon compound)

Cell Types for Photosynthesis

Only mesophyll cells

Mesophyll and bundle sheath cells

Environmental Adaptation

Prefer cool, wet climates

Adapted for hot, dry conditions

Abundance

About 95% of green plants

About 5% of green plants

Anatomy

No Kranz anatomy

Has Kranz anatomy

Chloroplast Types

Only granal chloroplasts

Both granal and agranal chloroplasts

Carbon Fixation Frequency

Fixes CO2 once

Fixes CO2 twice

Optimal Temperature

Lower optimum temperature for photosynthesis

Higher optimum temperature for photosynthesis

Photorespiration

Not suppressed

Suppressed

Stomatal Behavior

Photosynthesis occurs only when stomata are open

Can photosynthesize even when stomata are closed

Kranz Anatomy in C4 Plants

In leaves of C4 plants, each vascular bundle or rib is enclosed with a bundle sheath composed of larger parenchymatous cells. The bundle sheath cells have larger chloroplasts, lacking inter granal lamellae, and starch grains, while the mesophyll cells have small chloroplasts with grana. The anatomy is thus especially suited to increase carbon fixation efficiency due to the arrangement, so it is called Kranz anatomy from the German for "wreath".

Adaptive and Ecological Significance

The adaptations and ecological significance of the C3 and C4 plants include:

Parameter

C3 Plants

C4 Plants

Habitat preference

Temperate regions

Tropicl regions

Productivity

Lower

Higher

Drough resistance

Low

Hight

Water use

Comparatively less efficient

More efficient

C3 vs C4 Pathway NEET MCQs (With Answers & Explanations)

Important topics for NEET are:

  • C3 vs C4 Pathway

  • Kranz Anatomy

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Practice Questions for NEET

Q1. Phosphoenol pyruvate (PEP) is the primary CO2 acceptor in:

  1. C3 plants

  2. C4 plants

  3. C2 plants

  4. C3 and C4 plants

Correct answer: 2) C4 Plants

Explanation:

Phosphoenolpyruvate (PEP), a three-carbon chemical found in mesophyll cells, is the main CO2 acceptor.
Bicarbonate ions are the form in which the CO2 is received.
Bicarbonate is created when carbonic anhydrase hydrates CO2.
The PEP carboxylase or PEPcase enzyme is in charge of this fixing.
It is crucial to note that the RuBisCO enzyme is absent from mesophyll cells.
The mesophyll cells produce the C4 acid OAA.
Other 4-carbon compounds, such as aspartic acid or malic acid, are subsequently formed in the mesophyll cells and transferred to the bundle sheath cells.

Hence, the correct answer is option (2) C4 plants

Q2. Which of the following enzymes catalyses primary CO2 fixation in the C4 pathway?

  1. Alsolase

  2. PEP carboxylase

  3. RuBP carboxylase

  4. Isomerase

Correct answer: 2) PEP carboxylase

Explanation:

During the C4 pathway, CO2 combines with 3 the carbon compound phosphoenol pyruvate to form 4 the carbon compound oxaloacetic acid. This reaction is catalysed by PEP carboxylase. The formation of oxaloacetic acid occurs in the mesophyll cells and helps minimize photorespiration. Oxaloacetic acid is then converted into malate or aspartate, which is transported to bundle sheath cells for further processing. This adaptation allows C4 plants, such as maize and sugarcane, to maintain high photosynthetic efficiency in arid environments.

Hence, the correct answer is option 2) PEP carboxylase

Q3. Identify the incorrect statement in relation to C4 photosynthesis

  1. Kranz anatomy is an essential feature for c4 plants

  2. C4 plants have higher water use efficiency than C3 plants

  3. Photorespiration can be minimized when C4 the pathway is in operation

  4. Conversion of oxaloacetate to malate occurs in the bundle sheath cells

Correct answer: 4) Conversion of oxaloacetate to malate occurs in the bundle sheath cells

Explanation:

The mesophyll cells, not the bundle sheath cells, in C4 plants are where oxaloacetate is converted to malate. After that, malate is sent to the bundle sheath cells, which release CO₂ in preparation for the Calvin cycle. C4 plants have Kranz anatomy, which aids in the fixation of CO2. Compared to C3 plants, C4 plants use water more efficiently. Photorespiration is reduced via the C4 pathway.

Hence, the correct answer is option 4) Conversion of oxaloacetate to malate occurs in the bundle sheath cells.

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Frequently Asked Questions (FAQs)

Q: Where are C3 plants commonly found?
A:

C3 plants are usually found in cool and wet environments such as temperate climates.

Q: What is Kranz's anatomy?
A:

C4 plants have a specialized leaf anatomy called Kranz anatomy, which provides higher efficiency in carbon fixation. 

Q: How does photorespiration affect C3 plants?
A:

In C3 plants, photorespiration is not suppressed; the fixed carbon is wasted in this process, and thus photosynthetic efficiency decreases.

Q: What are C3 plants?
A:

C3 plants are plants wherein 3-phosphoglycerate, or 3-PGA, a 3-carbon compound, is the initial product of photosynthesis.

Q: What are C4 plants?
A:

C4 plants are plants that use the C4 photosynthetic pathway to produce a 4-carbon compound, namely oxaloacetate.

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