Difference between Prokaryotic and Eukaryotic Cells

Difference between Prokaryotic and Eukaryotic Cells

Irshad AnwarUpdated on 02 Jul 2025, 05:57 PM IST

Cells are the fundamental units of life, categorised into two main types: asexual and the two broad categories of cells, namely the prokaryotic and eukaryotic types of cells. Thus, the bacteria and archaea, called prokaryotic cells, do not contain a nucleus and membrane-bound organelles, while the plant, animal, and fungi cells and even some animal-like protists, called eukaryotic cells, contain these structures. This knowledge of cellular differences is crucial for biology students and applies to medicine, research, and biotechnology.

The organism is a collection of a population of cells, and each cell has having independent capacity to perform basic activities such as metabolic and reproductive mechanisms. Prokaryotic cells are much smaller and comparatively simpler not having a nuclear membrane and organelia while eukaryotic cells are larger and structurally complex; they contain a nuclear membrane along with several organelia each having a specific function. These differences show the variety of life forms and show how organisms can live in certain conditions/under certain conditions.

Detailed Comparison Table: Prokaryotic vs. Eukaryotic Cells

This comparison talks about the differences between prokaryotic and eukaryotic cells, providing an overview of their structures and functions.

Feature

Prokaryotic Cells

Eukaryotic Cells

Capsule

Present in some bacteria for protection and adhesion

Generally absent, though some eukaryotes have similar structures (e.g., slime layers)

Nucleus

Absent; DNA in nucleoid region

Present; DNA enclosed within a nuclear envelope

Size

Typically 0.1-5.0 µm

Typically 10-100 µm

Organelles

No membrane-bound organelles

Membrane-bound organelles present (e.g., mitochondria, ER, Golgi apparatus)

DNA Structure

Circular DNA

Linear DNA

Ribosome Size

70S (smaller)

80S (larger)

Cell Division

Binary fission

Mitosis and meiosis

Cell Wall

Peptidoglycan (bacteria), varied in archaea

Cellulose (plants), chitin (fungi), absent in animal cells

Membrane-bound Nucleus

Absent

Present

Cytoskeleton

Simple structures

Complex network of microtubules, microfilaments, and intermediate filaments

Mitochondria

Absent

Present

Golgi Apparatus

Absent

Present

Lysosomes

Absent

Present

Peroxisomes

Absent

Present

Vacuoles

Small or absent

Large central vacuole in plants; small vacuoles in animals

Chloroplasts

Absent

Present in plants and some protists

Pili/Fimbriae

Present for adhesion and conjugation

Absent

Cilia

Absent

Present in some eukaryotic cells for movement and sensory functions

Cytoplasmic Streaming

Absent

Present in plant and fungal cells

Photosynthetic Apparatus

Present in some (e.g., cyanobacteria)

Present in chloroplasts of plants and algae

Introns in Genes

Rare

Common

Histones Associated with DNA

Absent (except in some archaea)

Present

RNA Processing

Minimal

Extensive (capping, splicing, polyadenylation)

Plasmids

Common

Rare, but present in some cases (e.g., yeast)

Reproduction

Asexual, primarily binary fission

Sexual and asexual, involving mitosis and meiosis


Prokaryotic Cells

In the previous discussion of the derivation of the term "prokaryote," it is now understood that "pro" is derived from the Greek word meaning before, and "karyon," meaning nut or kernel, which refers to the nucleus. Therefore, “prokaryote” can be translated as “before the nucleus,” and this points to the fact that such organisms do not possess a real nucleus.

It is a category of the simplest cells, mostly unicellular organisms with no membrane-bound nucleus or membrane-bounded organelle. They are characterized by their simple structure, which includes: They are characterised by their simple structure, which includes:

  • Simple structure: Prokaryotic cells are usually smaller and less complex than eukaryotic cells; they are not enclosed by a membrane.
  • Lack of nucleus: The DNA is not surrounded by a nuclear membrane, but it is located at a particular site in the cell known as a nucleoid.
  • Absence of membrane-bound organelles: Cell organelles or structures like mitochondria, endoplasmic reticulum, Golgi apparatus, etc., are not found in prokaryotic cells.

Commonly Asked Questions

Q: What is the difference in the presence of plasmids between prokaryotic and eukaryotic cells?
A:
Plasmids, small circular DNA molecules separate from the main chromosome, are common in prokaryotic cells. They are rare in eukaryotic cells, mainly found in some lower eukaryotes like yeast.
Q: What is the difference in protein synthesis locations between prokaryotic and eukaryotic cells?
A:
In prokaryotic cells, protein synthesis occurs in the cytoplasm as soon as mRNA is produced. In eukaryotic cells, transcription (mRNA production) occurs in the nucleus, while translation (protein synthesis) happens in the cytoplasm or on the rough endoplasmic reticulum.
Q: What is the difference in RNA processing between prokaryotic and eukaryotic cells?
A:
In prokaryotic cells, mRNA is used for translation immediately after transcription. In eukaryotic cells, mRNA undergoes extensive processing in the nucleus, including splicing to remove introns, before being transported to the cytoplasm for translation.
Q: What is the difference in the location of DNA replication between prokaryotic and eukaryotic cells?
A:
In prokaryotic cells, DNA replication occurs in the cytoplasm. In eukaryotic cells, DNA replication takes place within the nucleus.
Q: How do prokaryotic and eukaryotic cells differ in their ability to perform cellular respiration?
A:
Both cell types can perform cellular respiration, but eukaryotic cells have specialized organelles (mitochondria) for this process. Prokaryotic cells perform respiration using their cell membrane or through enzymes in the cytoplasm.

Detailed Structure of Prokaryotic Cells

The following describes the detailed structure of prokaryotic cells:-

  • Cell Membrane: The prokaryotic cell membrane or the cell membrane is made of the phospholipid layer that regulates the movement in and out of the cell substances to support homeostasis.
  • Cell Wall: In bacteria, the cell wall is mainly constituted by peptidoglycan which plays the role of giving mechanical support as well as a protection function. In archaea, the composition may differ, but it doesn’t have peptidoglycan inside of it.
  • Nucleoid: The organelle is nucleoid which is an irregular structure within the cell housing the genetic material of the cell. It does not develop a membrane that surrounds it.
  • Cytoplasm: Cytoplasm can be described as a jelly-like material of the cell that consists of water, enzymes, nutrients, waste products, and gases. It contains the ribosomes and is the location of most of the cellular activities.
  • Ribosomes: Prokaryotic ribosomes are of smaller size compared to the eukaryotic ribosomal ribosomes and have a sedimentation value of 70S, which is made up of 50S and 30S parts. They are involved in the topology of hair and are also involved in protein synthesis.
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External Structures

  • Flagella: Sensory and locomotive appendages in the form of long whips.
  • Pili and Fimbriae: It is used in attachment to various surfaces and conjugation-like hair-like structures.
  • Capsule: An outer skin-like structure that gives a protective tone as well as outer layer adhesion.

Functions of Prokaryotic Cells

  • Binary fission: The application of a type of asexual reproduction whereby a cell of an organism splits into two genetically similar cells.
  • Metabolism: Prokaryotic cells have multiple strategies of energy metabolism that are known as aerobic and anaerobic respiration, fermentations, and photosynthesis.
  • Genetic variation: It is done through conjugation (transfer of genetic material between bacteria), transformation (taking of foreign DNA), and transduction which is the transferring of genetic material through a bacteria virus.

Examples of Prokaryotic Cells

  • Bacteria: Some of the examples include Escherichia coli (E. coli) and Staphylococcus aureus.
  • Archaea: Some examples that can be taken under this category are Methanogens and Halophiles.


Commonly Asked Questions

Q: How do prokaryotic and eukaryotic cells differ in their ability to compartmentalize cellular processes?
A:
Eukaryotic cells can compartmentalize various cellular processes in different organelles, allowing for more efficient and specialized functions. Prokaryotic cells, lacking membrane-bound organelles, perform most cellular processes in the cytoplasm.
Q: What are ribosomes, and how do they differ between prokaryotic and eukaryotic cells?
A:
Ribosomes are cellular structures responsible for protein synthesis. While both cell types have ribosomes, prokaryotic ribosomes are smaller (70S) compared to eukaryotic ribosomes (80S). Eukaryotic cells also have ribosomes attached to the rough endoplasmic reticulum.
Q: How do prokaryotic and eukaryotic cells differ in their chromosome number?
A:
Prokaryotic cells typically have a single circular chromosome. Eukaryotic cells usually have multiple linear chromosomes, with the number varying among species (e.g., humans have 23 pairs).
Q: What is the difference in cell membrane composition between prokaryotic and eukaryotic cells?
A:
While both cell types have phospholipid bilayer membranes, eukaryotic cell membranes contain cholesterol, which provides additional stability. Prokaryotic cell membranes lack cholesterol but may contain other sterols.
Q: What is the difference in the cytoskeleton structure between prokaryotic and eukaryotic cells?
A:
Eukaryotic cells have a well-developed cytoskeleton composed of microfilaments, intermediate filaments, and microtubules. Prokaryotic cells have cytoskeletal elements, but they are less complex and diverse than those in eukaryotes.

Eukaryotic Cells

  • About the term “eukaryote”

The term eukaryote is derived from the Greek words 'eu' (true) and 'karyon' (nut or kernel), which refer to the nucleus. Therefore, such organisms possess a membrane-bound nucleus

  • Definition and Characteristics

Eukaryotic cells are complex, multicellular, or unicellular organisms characterised by the following features:

  • Complex structure: Rather than having a simple structure, eukaryotic cells are a lot bigger, having a complex internal structure with one or many organelles depending on the cell's functions.
  • Presence of a true nucleus: The nucleus is surrounded by a double membrane called the nuclear envelope which is located outside the nucleus.
  • Membrane-bound organelles: Some of the structures found at the sub-cellular level including the mitochondria endoplasmic reticulum and the Golgi apparatus are enclosed in a membrane.
  • Detailed Structure of Eukaryotic Cells
  • Nucleus: The nuclear envelope is a two-layer membrane that surrounds the nucleus and has openings referred to as nuclear pores. The nucleolus is the part of the nucleus where ribosome is synthesized. Chromatin contains the DNA and protein that condenses to form chromosomes during cell division.

Diagram: Structure of a Nucleus

1723466948656

  • Cell Membrane: The cell membrane as a protective barrier is composed of phospholipid layered that has proteins that regulate the transport of molecules in and out of a cell along with acting as receptors.

Diagram: Cell Membrane Structure

1723466949157

  • Cytoplasm and Cytoskeleton: Cytoplasm is a semi-fluid mass in the cell composed of water, food materials, and any other structures in the cell. The cytoskeleton is composed of protein fibers (microfilament, intermediate filament, micro thread) that give structural support, shape cytoplasm, and well as movement.
  • Organelles

Mitochondria: Mitochondria of the cell, synthesize ATP through the process of cell respiration. These organisms have a double membrane and their DNA.

Endoplasmic Reticulum: Ribosome at studded on the rough endoplasmic reticulum; manufactures and modifies proteins

Golgi Apparatus: Golgi apparatus changes and sorts proteins and lipids, and packs them for transportation

Lysosomes and Peroxisomes: Lysosomes have enzymes for breaking down macromolecules that are present in the food ingested by the organism. Peroxisomes Emulsify fats and foreign materials as well as perform the detoxifying function

Vacuoles: Big and membrane-bound structures consisting of reserve foods, and wastes, and mainly involved in the osmotic potential of a plant cell.

Chloroplasts (in plant cells): Sources of photosynthesis, include chlorophyll and a double membrane.

Diagram: Various Organelles in Eukaryotic Cells

1723466948307



  • Functions of Eukaryotic Cells

  • Cell division: Mitosis is the process of the formation of two alike daughter cells from one mother cell. Meiosis is the division that yields four genetically different daughter cells, which produce gametes.
  • Metabolism: Cellular respiration is the procedure of carrying out oxidative phosphorylation to generate ATP from glucose in the mitochondria. Photosynthesis is the conversion of light energy into chemical energy in the chlorophyll-containing pigmented organelles known as chloroplasts in plant cells.
  • Protein synthesis: Transcribing DNA to mRNA in the nucleus. At the ribosome, proteins are made using the information coded by the mRNA.
  • Cell signaling: Structures that comprise cells, tissues, and organs, which play a role in summoning other cells and molecules as well as in the overall regulation of cellular activities and the organism’s interaction with the surroundings.
  • Examples of Eukaryotic Cells
  • Animals: Singly and multiply organized forms of life such as human beings, and animals.
  • Plants: Complex forms of organisms commonly include; trees and flowers.
  • Fungi: Yeasts, moulds, mushrooms.
  • Protists: organisms such as amoebas, algae, or noncomplex organisms with one cell or a few cells.

Commonly Asked Questions

Q: What is the fundamental difference between prokaryotic and eukaryotic cells?
A:
The fundamental difference is the presence of a membrane-bound nucleus. Prokaryotic cells lack a true nucleus, with their genetic material floating freely in the cytoplasm. Eukaryotic cells have a distinct nucleus enclosed by a nuclear membrane, which houses and protects their DNA.
Q: How do prokaryotic and eukaryotic cells differ in their genetic material organization?
A:
In prokaryotic cells, the genetic material (DNA) is typically a single circular chromosome located in the nucleoid region. Eukaryotic cells have multiple linear chromosomes contained within the nucleus, along with histone proteins that help package the DNA.
Q: How do prokaryotic and eukaryotic cells differ in terms of internal compartmentalization?
A:
Prokaryotic cells have a simpler internal structure with no membrane-bound organelles. Eukaryotic cells have numerous membrane-bound organelles such as mitochondria, endoplasmic reticulum, and Golgi apparatus, which compartmentalize various cellular functions.
Q: What is the cell wall composition difference between prokaryotic and eukaryotic cells?
A:
Prokaryotic cells typically have a cell wall made of peptidoglycan. Eukaryotic cell walls, when present (e.g., in plant cells), are usually composed of cellulose. Many eukaryotic cells, like animal cells, lack a cell wall entirely.
Q: How do prokaryotic and eukaryotic cells differ in their ability to perform photosynthesis?
A:
Both types can perform photosynthesis, but eukaryotic cells (like plants and algae) use specialized organelles called chloroplasts. Photosynthetic prokaryotes (like cyanobacteria) use thylakoid membranes in the cytoplasm.

Frequently Asked Questions (FAQs)

Q: What is the difference in the presence of histones between prokaryotic and eukaryotic cells?
A:
Eukaryotic cells have histones, proteins that help package DNA into chromatin. Prokaryotic cells generally lack histones, although some archaea have histone-like proteins.
Q: How do prokaryotic and eukaryotic cells differ in their ability to perform signal transduction?
A:
Both cell types can perform signal transduction, but eukaryotic cells have more complex signaling pathways involving multiple organelles. Prokaryotic signaling is generally simpler and occurs mainly at the cell membrane.
Q: What is the difference in the presence of a cell cortex between prokaryotic and eukaryotic cells?
A:
Eukaryotic cells often have a cell cortex, a specialized layer of cytoplasm just beneath the plasma membrane. Prokaryotic cells lack this specialized cortex structure.
Q: How do prokaryotic and eukaryotic cells differ in their ability to perform protein folding?
A:
Eukaryotic cells have specialized compartments like the endoplasmic reticulum for protein folding and can use chaperone proteins. Prokaryotic cells perform protein folding in the cytoplasm, with a more limited set of chaperone proteins.
Q: What is the difference in the presence of telomeres between prokaryotic and eukaryotic cells?
A:
Eukaryotic cells have telomeres, repetitive DNA sequences at the ends of linear chromosomes that protect them from degradation. Prokaryotic cells, with their circular chromosomes, do not have telomeres.
Q: How do prokaryotic and eukaryotic cells differ in their ability to perform glycolysis?
A:
Both cell types can perform glycolysis, but in eukaryotic cells, it occurs in the cytoplasm. In prokaryotic cells, glycolysis and other metabolic processes occur in the cytoplasm without compartmentalization.
Q: What is the difference in the presence of a nuclear pore complex between prokaryotic and eukaryotic cells?
A:
Eukaryotic cells have nuclear pore complexes in their nuclear membrane, regulating the transport of molecules between the nucleus and cytoplasm. Prokaryotic cells lack a nuclear membrane and thus have no nuclear pore complexes.
Q: What is the difference in the presence of a Golgi apparatus between prokaryotic and eukaryotic cells?
A:
Eukaryotic cells have a Golgi apparatus, an organelle involved in modifying, sorting, and packaging proteins for secretion or use within the cell. Prokaryotic cells lack a Golgi apparatus.
Q: What is the difference in the presence of a nucleolus between prokaryotic and eukaryotic cells?
A:
Eukaryotic cells have a nucleolus, a structure within the nucleus where ribosomal RNA is synthesized and ribosomal subunits are assembled. Prokaryotic cells lack a nucleolus.
Q: How do prokaryotic and eukaryotic cells differ in their ability to perform meiosis?
A:
Eukaryotic cells can undergo meiosis, a specialized type of cell division that produces gametes with half the normal chromosome number. Prokaryotic cells do not perform meiosis and reproduce asexually.