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Ammonification - Overview, Examples, Defination

Ammonification - Overview, Examples, Defination

Edited By Irshad Anwar | Updated on Aug 20, 2025 04:58 PM IST

Ammonification is a crucial step of the nitrogen cycle where decomposers convert organic nitrogen into ammonia (NH₃) or ammonium ions (NH₄⁺). It helps recycle nutrients, improve soil fertility, and support plant growth. Learn its process, role, and NEET exam relevance.

Nitrogen is part of amino acids, proteins, and nucleic acids and is often a limiting nutrient in plants. Plants can use two inorganic forms of nitrogen: ammonium (NH₄⁺) and nitrate (NO3), and some organic forms, such as amino acids. The main reservoir of nitrogen is the atmosphere, which is 80% nitrogen gas (N2). The major pathway for nitrogen to enter an ecosystem is via nitrogen fixation. The conversion of N2 by bacteria to forms that can be used to synthesize nitrogenous organic compounds.

This Story also Contains
  1. What is Ammonification?
  2. Ammonification in the Nitrogen Cycle
  3. Process Of Ammonification
  4. Importance Of Ammonification for Soil Fertility
  5. Ammonification in Agriculture and Composting
  6. Ammonification vs Other Nitrogen Cycle Processes
  7. Weightage and Questions Types
  8. Ammonification NEET MCQs
  9. FAQs on Ammonification
  10. Recommended Video On Ammonification
Ammonification - Overview, Examples, Defination
Ammonification

Ammonification is the stage of the nitrogen cycle in which decomposers like bacteria and fungi convert dead organic nitrogen into ammonia (NH3) or ammonium ions (NH4⁺). This process is vital in the nitrogen cycle because it helps ensure that nutrients are recycled back into a form available to plants, thereby maintaining mineral nutrition.

What is Ammonification?

Ammonification is the breakdown of organic matter by decomposers, primarily bacteria or fungi, inside dead plants or animals, and then converting it back into ammonia (NH3) or ammonium ions (NH₄⁺). The recycling of nitrogen in the ecosystem is thus again taken up by this process, as it ensures that nitrogen enters the soil in a form useful for the plants, and it will continue to make the soil healthier and fertile.

Ammonification can be simply defined as the breakdown of organic nitrogen into ammonia, which reintroduces the nitrogen in the soil for further absorption by plants.

Ammonification in the Nitrogen Cycle

The other critical step of the nitrogen cycle is ammonification. It is the process of movement of nitrogen between the atmosphere, soil, and organisms through biogeochemical cycles. The absence of ammonification would mean an inability to use the nitrogen trapped in organic matter for growing plants and, hence, breaking ecosystems.

Process Of Ammonification

The ammonification process begins with dead plant and animal matter, and excreted wastes. Here is the procedure divided into individual steps:

Step 1: Decomposition of Organic Nitrogen

  • When plants and animals die, decomposer organisms, mostly bacteria and fungi, feed on the dead organic matter.

  • These organisms metabolize nitrogen-containing compounds such as proteins, nucleic acids, and urea, whereby they release ammonia (NH₃) or ammonium ions (NH₄⁺).

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Step 2: Conversion to Ammonia

Decomposer bacteria convert organic nitrogen compounds to ammonia. By undergoing enzymatic reactions, the chemical equation for ammonification is as follows:

Proteins + Water through enzyme activity → Amino acids

Amino acids through microbial action → Ammonia (NH₃) or Ammonium ions (NH₄⁺)

Step 3: Emission Of Ammonia

Once the ammonia is formed, it may stay as ammonium ions (NH₄⁺) in the soil, if the soil is acidic or it diffuses into the atmosphere as ammonia gas (NH₃).

Ammonification Reaction

A simple equation can be represented as:

Organic Nitrogen (Proteins/Dead Material) → Bacteria → Ammonia (NH₃) + Water (H2O).

Ammonium ions (NH₄⁺) are either absorbed directly or further processed through nitrification, converting it to nitrates (NO₃⁻), a readily available source of nitrogen for plants.

Importance Of Ammonification for Soil Fertility

It is crucial for the overall health and fertility of soil. It is involved in recycling nitrogen from dead organic matter that otherwise remains locked and unavailable for plant growth. It also keeps the nitrogen cycle running, a necessity to keep ecosystems balanced.

Ammonification in Agriculture and Composting

  • Ammonification replenishes the nitrogen source in soil, which is essential for plant growth and crop production improvement.

  • Organic fertilizers rely on the process of ammonification to replenish the nitrogen source in soil throughout various agricultural farms.

  • A good example of ammonification in action is in composting. Organic wastes such as decaying plant material, kitchen wastes, and manure from animals decompose with ammonification.

  • This converts the nitrogen of these materials into ammonia which can then be absorbed by the plants for growth improving soil fertility.

Diagram of ammonification showing decomposition of plants and animals by soil bacteria, leading to the formation of ammonium ions (NH₄⁺)

Ammonification vs Other Nitrogen Cycle Processes

Process

Description

Key Organisms

Nitrogen Fixation

Conversion of atmospheric nitrogen (N2) into ammonia (NH3) by nitrogen-fixing bacteria

Rhizobium, Azotobacter

Nitrification

Conversion of ammonia into nitrites (NO2-) and then into nitrates (NO3-) by nitrifying bacteria

Nitrosomonas, Nitrobacter

Ammonification

Decomposition of organic matter into ammonia by decomposers

Decomposer bacteria, fungi

Denitrification

Conversion of nitrates back into atmospheric nitrogen (N2)

Denitrifying bacteria


Weightage and Questions Types

Exam Type

Weightage of Ammonification

Types of Questions

CBSE Board Exams

4-6%

Definitions, role in the nitrogen cycle, examples

NEET

1-2%

MCQs, assertion-reason questions, reaction-based questions

Nursing Entrance Exams

2-3%

True/false, scenario-based questions related to soil health

Paramedical Entrance Exams

1-2%

Case studies on soil nitrogen management


Ammonification NEET MCQs

Q1. The correct sequence of stages in nitrogen fixation is

  1. Ammonification, nitrification and denitrification

  2. Nitrification, denitrification and ammonification

  3. Denitrification, nitrification and ammonification

  4. Ammonification, denitrification and nitrification

Correct answer: 1) Ammonification, nitrification and denitrification

Explanation:

The correct order of steps in nitrogen fixation is:
Nitrogen Fixation Nitrogen-fixing bacteria convert the atmospheric nitrogen to ammonia (NH₃).
Nitrification: Nitrifying bacteria break down ammonia in the following manner: oxidation into nitrites to nitrates.
Assimilation: Plants absorb nitrates and assimilate them into organic compounds.
Ammonification: Organic nitrogen is converted back into ammonia by decomposers.
Denitrification: Nitrates are reduced to nitrogen gas (N₂), returning it to the atmosphere.

Hence the correct answer is Option (1) Ammonification, nitrification and denitrification.

Q2. Decomposition of organic nitrogen of dead plants and animals into ammonia is called

  1. Nitrogen fixation

  2. Nitrification

  3. Nitration

  4. Ammonification

Correct answer: 4) Ammonification

Explanation:

The decomposition of organic matter containing nitrogen, such as decaying dead plants and animals, forms ammonia. In such a microbially-based process, decomposers break down proteins and nucleic acids that happen to contain nitrogen and consequently release ammonia in the soil. Released ammonia is taken by the plants or continues further conversion into nitrates during nitrification and serves as an integral part of the nitrogen cycle.

Hence, the correct answer is option 4) Ammonification.

Q3. Plants accumulate _________in the form of ammonium

  1. Oxygen

  2. Carbon

  3. Nitrogen

  4. All of the above

Correct answer: 3) Nitrogen

Explanation:

Plants accumulate nitrogen in the form of ammonium. The reduction of atmospheric nitrogen to ammonia is called nitrogen fixation. Higher plants generally utilize the oxidized forms such as nitrate (NO3-) and nitrite (NO2-) or the reduced form (N) of nitrogen, which is made available by a variety of nitrogen fixers. Nitrogen is a constituent of amino acids, proteins, hormones, chlorophyll, and many vitamins.

Hence, the correct answer is option 3) Nitrogen.

Also Read:

FAQs on Ammonification

Define ammonification.

Ammonification is the biological process in which organic nitrogen compounds present in dead plants, animals, and waste products are converted into ammonia (NH₃) or ammonium ions (NH₄⁺). This conversion is carried out by decomposer organisms. It is a crucial step of the nitrogen cycle because it transforms complex organic nitrogen into a simpler form that can re-enter soil nutrient pools. Without ammonification, nitrogen would remain locked in organic matter and unavailable to plants.

Which organisms carry out ammonification?

Ammonification is mainly carried out by decomposer microorganisms, especially bacteria and fungi. Examples include species of Bacillus, Pseudomonas, and fungi like Aspergillus and Penicillium. These organisms break down proteins, nucleic acids, and other nitrogenous wastes into ammonia. Thus, they act as recyclers in the ecosystem, ensuring a continuous supply of nitrogen for plant uptake.

What is the role of ammonification in the nitrogen cycle?

The role of ammonification is to recycle nitrogen locked in organic matter back into the ecosystem. By converting dead organic nitrogen into ammonia or ammonium ions, it replenishes the soil with nitrogen in a form that plants can directly absorb. This ensures soil fertility, supports plant growth, and maintains the balance of mineral nutrition. Without ammonification, nitrogen would accumulate in dead biomass and disrupt the nitrogen cycle.

Give an example of ammonification.

A common example of ammonification is the composting process. When plant residues, food scraps, or animal wastes are decomposed by microbes, the nitrogen present in proteins and other organic compounds is broken down and released as ammonia. This ammonia enriches the compost, making it a nitrogen-rich manure. Such natural recycling of nitrogen helps in sustainable agriculture and soil fertility management.

Recommended Video On Ammonification


Frequently Asked Questions (FAQs)

1. Why ammonification is an important process?

Ammonification Definition:Ammonification is the process of converting organic nitrogen from dead organisms to ammonia by microorganisms.

Ammonification Importance

It provides essential nitrogen for plants that do not have a symbiotic relationship.

It maintains the ecosystem by recycling the dead organisms organic nitrogen compounds such as amino acids,dna,urea.

It balances the nitrogen in the atmosphere and the ecosystem .

It is crucial as it converts organic nitrogen into inorganic nitrogen ammonia.

2. Which is the abundant gas in the atmosphere?

Nitrogen is the abundant gas in the atmosphere which makes78% of air.It is essential component for proteins,dna.It is required for growth,metabolic process and reproduction.Nitrogen is converted to ammonia and nitrates by nitrifying and ammonifying bacteria.So that plants can access it.

3. What is the difference between ammonification and nitrification?

ammonification-It is the process of conversion of organic nitrogen(dead organisms,feces,urea)  into ammonia by bacteria or fungi.they break down dead organisms.

Nitrification-Oxidation of ammonium ions into nitrate by nitrifying bacteria.nitrate is assimilated by plants. 

4. Where are ammonifying bacteria found?

Symbiotic bacteria(depending on other organisms) are found at the root nodules of plants and they take shelter from plants by providing nitrogen to plants through conversions.

Non symbiotic bacteria(not dependent on other organisms) live freely and they fix nitrogen for their own  purpose.

5. What is the difference between ammonification and ammonium assimilation?

Ammonification is the production of ammonia from organic nitrogen compounds, while ammonium assimilation is the process by which organisms incorporate ammonium ions into organic compounds, such as amino acids. Ammonification releases nitrogen, while assimilation consumes it.

6. What is the role of ammonification in nitrogen retention in ecosystems?

Ammonification plays a crucial role in nitrogen retention by converting organic nitrogen into forms that can be readily absorbed by plants or further transformed in the nitrogen cycle. This process helps prevent nitrogen loss through leaching or gaseous emissions, maintaining the nitrogen pool within the ecosystem.

7. How does ammonification affect plant species composition in natural ecosystems?

Ammonification can influence plant species composition by affecting the availability and form of nitrogen in the soil. Some plant species prefer ammonium as their nitrogen source, while others prefer nitrate. The rate and efficiency of ammonification can thus favor certain plant species over others, influencing community structure.

8. How does ammonification differ from nitrogen fixation?

Ammonification converts organic nitrogen into ammonia, while nitrogen fixation converts atmospheric nitrogen (N2) into ammonia. Ammonification is performed by various microorganisms, whereas nitrogen fixation is carried out by specific bacteria, such as Rhizobium in legume root nodules.

9. What is the difference between ammonification and mineralization?

Ammonification is a specific type of mineralization that focuses on the conversion of organic nitrogen to ammonia or ammonium. Mineralization, in a broader sense, refers to the conversion of any organic nutrient into its inorganic form, not just nitrogen.

10. What happens to the ammonia produced during ammonification?

The ammonia produced during ammonification can follow several paths: it may be absorbed directly by plants, converted to ammonium ions in acidic soils, undergo nitrification to form nitrates, or be lost to the atmosphere through volatilization, especially in alkaline soils.

11. What is the relationship between ammonification and decomposition?

Ammonification is an integral part of the decomposition process. As microorganisms break down organic matter (decomposition), they release nitrogen in the form of ammonia (ammonification). Both processes work together to recycle nutrients in the ecosystem.

12. How does ammonification affect soil pH?

Ammonification can temporarily increase soil pH due to the production of ammonia, which is basic. However, the subsequent conversion of ammonia to ammonium ions (NH4+) in soil water can lead to a slight decrease in pH as hydrogen ions (H+) are released.

13. What is the chemical equation for the ammonification of a simple amino acid?

The ammonification of a simple amino acid, such as glycine, can be represented by the following equation:

14. How do earthworms contribute to ammonification?

Earthworms contribute to ammonification indirectly by enhancing microbial activity in the soil. They break down organic matter, increase soil aeration, and create nutrient-rich casts, all of which promote the growth and activity of ammonifying microorganisms.

15. What is the role of ammonification in composting?

In composting, ammonification is a key process that breaks down nitrogen-containing organic compounds in plant and animal waste. It releases ammonia, which can either be incorporated into microbial biomass or lost through volatilization. Proper management of the composting process aims to maximize nitrogen retention through ammonification.

16. How do freeze-thaw cycles affect ammonification in soil?

Freeze-thaw cycles can stimulate ammonification by physically breaking down organic matter and lysing microbial cells, releasing their contents. This process, known as the "freeze-thaw effect," can lead to pulses of ammonification and nitrogen availability in soils subject to seasonal freezing.

17. How does ammonification contribute to eutrophication in water bodies?

Ammonification can contribute to eutrophication when excess organic matter in water bodies leads to increased ammonia production. This ammonia can be converted to nitrates, fueling algal blooms. Additionally, high ammonia levels can be directly toxic to aquatic organisms.

18. Why is ammonification important for plant growth?

Ammonification is essential for plant growth because it converts organic nitrogen, which plants cannot directly use, into inorganic forms (ammonia or ammonium) that plants can absorb and utilize for various metabolic processes, including protein synthesis and chlorophyll production.

19. How does ammonification contribute to soil fertility?

Ammonification contributes to soil fertility by converting organic nitrogen into plant-available forms (ammonia and ammonium). This process helps maintain a steady supply of nitrogen for plant growth and supports the overall nutrient cycling in the soil ecosystem.

20. How does ammonification relate to the concept of nutrient cycling?

Ammonification is a key step in the nitrogen cycle, one of the most important nutrient cycles. It transforms organic nitrogen back into inorganic forms, making it available for plant uptake and other processes, thus ensuring the continuous cycling of nitrogen in ecosystems.

21. What is the role of ammonification in aquatic ecosystems?

In aquatic ecosystems, ammonification plays a crucial role in recycling nitrogen from dead organisms and waste products. It occurs in sediments and water columns, providing a source of nitrogen for aquatic plants and algae. However, excessive ammonification can lead to water quality issues.

22. What is the significance of ammonification in forest ecosystems?

In forest ecosystems, ammonification is crucial for recycling nitrogen from leaf litter and dead wood. It helps maintain soil fertility, supports tree growth, and contributes to the overall nitrogen budget of the forest. The process is particularly important in nitrogen-limited forest ecosystems.

23. What is the difference between ammonification and putrefaction?

While both processes involve the breakdown of organic matter, ammonification specifically refers to the conversion of organic nitrogen to ammonia. Putrefaction is a more general term for the decomposition of proteins by certain bacteria, often associated with foul odors. Ammonification is a component of putrefaction but not all putrefaction involves ammonification.

24. What are the main sources of organic nitrogen for ammonification?

The main sources of organic nitrogen for ammonification include dead plant and animal tissues, animal waste, and other organic matter in the soil. These materials contain nitrogen-rich compounds like proteins and nucleic acids that are broken down during ammonification.

25. What is the role of enzymes in the ammonification process?

Enzymes play a crucial role in ammonification by catalyzing the breakdown of complex organic nitrogen compounds. Key enzymes include proteases, which break down proteins into amino acids, and deaminases, which remove amino groups from amino acids to form ammonia.

26. Which microorganisms are primarily responsible for ammonification?

Ammonification is primarily carried out by heterotrophic bacteria and fungi in the soil. These microorganisms break down organic matter containing nitrogen, such as dead plant and animal tissues, and release ammonia as a byproduct of their metabolism.

27. How does soil moisture content affect ammonification?

Soil moisture content plays a crucial role in ammonification. Moderate soil moisture (50-60% of water-holding capacity) is optimal for the process. Too little water can limit microbial activity, while excessive moisture can create anaerobic conditions that slow down ammonification.

28. How does the carbon-to-nitrogen (C:N) ratio of organic matter affect ammonification?

The C:N ratio of organic matter significantly influences ammonification. Materials with a low C:N ratio (below 20:1) tend to undergo rapid ammonification, releasing excess nitrogen. High C:N ratios (above 30:1) can lead to nitrogen immobilization, where microbes use available nitrogen for their own growth.

29. How does salinity affect the ammonification process?

High soil salinity can inhibit ammonification by reducing microbial activity and altering the soil microbial community composition. Excessive salt concentrations can cause osmotic stress in microorganisms, slowing down their metabolic processes, including ammonification.

30. What is the impact of acid rain on ammonification processes?

Acid rain can negatively impact ammonification by lowering soil pH, which can reduce the activity of ammonifying microorganisms. However, the increased nitrogen deposition associated with acid rain can also stimulate microbial activity. The net effect depends on the specific ecosystem and its buffering capacity.

31. What is the relationship between ammonification and amino acid catabolism?

Ammonification is closely related to amino acid catabolism. When microorganisms break down amino acids for energy (catabolism), they often release the amino group as ammonia through a process called deamination. This deamination is a key step in the overall process of ammonification.

32. How does soil pH affect the ammonification process?

Soil pH can significantly influence ammonification. The process generally occurs more rapidly in slightly acidic to neutral soils (pH 6-7). Extremely acidic or alkaline conditions can inhibit the activity of ammonifying microorganisms, slowing down the process.

33. What is the impact of pesticides on ammonification?

Pesticides can negatively impact ammonification by reducing the population or activity of soil microorganisms responsible for the process. Some pesticides may directly inhibit enzymes involved in ammonification, while others may alter the soil microbial community structure.

34. How does soil texture affect ammonification rates?

Soil texture influences ammonification rates by affecting soil aeration, water retention, and microbial habitat. Sandy soils with good aeration tend to have higher ammonification rates, while clay soils may have slower rates due to reduced oxygen availability and potential waterlogging.

35. How does temperature affect the rate of ammonification?

Temperature significantly influences ammonification rates. The process generally increases with rising temperatures up to an optimal range (25-35°C). Extremely high or low temperatures can slow down or halt the process by affecting microbial activity.

36. What is the difference between ammonification and denitrification?

Ammonification converts organic nitrogen to ammonia, making nitrogen more available to plants. Denitrification, on the other hand, converts nitrates back to atmospheric nitrogen (N2), removing nitrogen from the soil ecosystem. Ammonification is carried out by various microbes in aerobic conditions, while denitrification occurs under anaerobic conditions.

37. How do agricultural practices affect ammonification rates in soil?

Agricultural practices can significantly impact ammonification rates. Tillage can temporarily increase rates by aerating soil and exposing organic matter. Fertilizer application can affect microbial communities and substrate availability. Crop rotation and cover cropping can influence the quantity and quality of organic matter available for ammonification.

38. How does the presence of heavy metals in soil affect ammonification?

Heavy metals can negatively impact ammonification by toxically affecting soil microorganisms. They can inhibit enzyme activities, reduce microbial biomass, and alter the composition of microbial communities responsible for ammonification. The severity of the impact depends on the type and concentration of heavy metals present.

39. How does ammonification differ in aerobic versus anaerobic environments?

Ammonification occurs more efficiently in aerobic environments where oxygen is readily available for microbial respiration. In anaerobic environments, ammonification still occurs but at a slower rate and may be coupled with fermentation processes. The end products and microbial communities involved can also differ between these conditions.

40. What is the impact of global warming on ammonification rates?

Global warming is likely to increase ammonification rates in many ecosystems due to higher temperatures stimulating microbial activity. However, this effect may be offset in some areas by decreased soil moisture. Changes in ammonification rates could have cascading effects on nitrogen cycling and ecosystem functioning.

41. What is the role of ammonification in the decomposition of animal carcasses?

Ammonification plays a significant role in the decomposition of animal carcasses by breaking down proteins and other nitrogen-containing compounds. This process releases ammonia, contributing to the characteristic odor of decomposition and recycling nitrogen back into the ecosystem.

42. What is the role of ammonification in the restoration of degraded soils?

Ammonification is crucial in the restoration of degraded soils as it helps rebuild soil organic matter and nitrogen content. By promoting the growth of vegetation through increased nitrogen availability, ammonification supports the overall process of soil rehabilitation and ecosystem recovery.

43. How does ammonification contribute to the nitrogen nutrition of mycorrhizal fungi?

Mycorrhizal fungi can benefit from ammonification by absorbing the released ammonium ions. Some mycorrhizal fungi can also directly participate in ammonification, breaking down organic nitrogen compounds in the soil. This process enhances nitrogen acquisition for both the fungi and their plant partners.

44. How does ammonification differ between terrestrial and aquatic ecosystems?

While the basic process is similar, ammonification in aquatic ecosystems often occurs in sediments or the water column, whereas in terrestrial ecosystems it primarily occurs in soil. Aquatic ammonification can lead to more immediate effects on water quality, while terrestrial ammonification is more closely tied to soil fertility and plant nutrition.

45. How does ammonification contribute to the formation of soil organic matter?

While ammonification itself breaks down organic matter, it also supports the formation of new soil organic matter. The ammonia released can be assimilated by microorganisms, contributing to microbial biomass. Dead microbial cells and their byproducts then become part of the soil organic matter pool.

46. What is the relationship between ammonification and the carbon cycle?

Ammonification is closely linked to the carbon cycle. As microorganisms break down organic matter for ammonification, they also release carbon dioxide through respiration. The rate of ammonification can thus influence carbon mineralization rates and soil carbon storage.

47. How does ammonification affect the availability of other nutrients in the soil?

Ammonification can indirectly affect the availability of other nutrients. The process of organic matter breakdown releases not only nitrogen but also other elements like phosphorus and sulfur. Additionally, the pH changes associated with ammonification can influence the solubility and availability of various soil nutrients.

48. How does ammonification contribute to the nitrogen cycle in desert ecosystems?

In desert ecosystems, ammonification is crucial for nitrogen cycling, despite occurring at lower rates due to limited organic matter and moisture. It often occurs in pulses following rare rain events, rapidly recycling nitrogen from dead organisms and helping to support the sparse vegetation.

49. How does ammonification relate to the concept of nutrient use efficiency in plants?

Ammonification contributes to nutrient use efficiency by recycling organic nitrogen into forms that plants can readily use. Plants that can effectively utilize ammonium produced by ammonification may have a competitive advantage, especially in ecosystems where this is the primary form of available nitrogen.

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