Cytoplasm: Structure and Function

What is Cytoplasm?

The cytoplasm is that part of a cell that lies between the plasma membrane and the nuclear envelope—everything within the membrane but outside the nucleus. The cytoplasm comprises mainly cytosol and organelles. Organelles are specialised structures that have characteristic shapes and perform specific functions in the cell.

In cell biology, the cytoplasm is important for maintaining the structural integrity of an individual cell, metabolic activities, and providing a space for intra-cellular transport. The cytoplasm provides a medium for the suspension of organelles and is involved in many of the activities of a cell, like growth, replication, and response to an external stimulus.

Structure of the Cytoplasm

The structure of cytoplasm consists of:

Basic Composition

The cytoplasm is a highly complex and dynamic medium structure of the cell, composed of various elements that act in concert to permit cellular integrity and the ability of the cell to perform a wide range of functions.

Cytosol (Intracellular Fluid)

• The cytosol is the semifluid portion of the cytoplasm. All the cellular processes take place in the cytosol. It is the matrix in which the various organelles are suspended.

• It consists mainly of water (about 70-80%), but also of dissolved ions, small molecules, and large water-soluble molecules (such as proteins).

• The cytosol is the site for a lot of metabolic pathways—for example, glycolysis—and has an important soft role in the transportation of metabolites and signalling molecules.

Organelles Suspended in the Cytoplasm

• Mitochondria: The mitochondria are the powerhouses of the cell. It generates ATP through the process of oxidative phosphorylation.

• Endoplasmic Reticulum (ER): Rough ER is so named because it is studded with ribosomes that are actively involved in protein synthesis and protein modification. The smooth ER does not have ribosomes and, hence, is not involved in protein synthesis. It involves lipid synthesis and detoxification processes.

• Golgi Apparatus: Modifies, sorts, and packages proteins and lipids for secretion or delivery to other organelles.

• Lysosomes: Contains hydrolytic enzymes for the digestion of macromolecules and worn-out organelles.

• Peroxisomes: Involved in fatty acid metabolism and the detoxification of harmful substances.

• Ribosomes: The sites of protein synthesis, either float freely in the cytoplasm or are attached to the rough ER.

Cytoskeleton

The cytoskeleton is a dynamic network of protein filaments within the cytoplasm, comprising microfilaments, intermediate filaments, and microtubules.

• Microfilaments, composed of actin, are involved in cell movement and shape maintenance.

• Intermediate filaments provide mechanical support and maintain cell integrity,

• Microtubules, made of tubulin, are essential for cell division, intracellular transport, and structural support.

Together, these components help maintain cell shape, facilitate intracellular transport by acting as tracks for organelle movement, and play a crucial role in chromosome separation during mitosis and meiosis.

Functions of the Cytoplasm

The cytoplasm has specialised regions to perform certain functions instead of well defined organelles. The main functions of cytoplasm in a prokaryotic cell are:

Support and Suspension of Organelles

• The cytoplasm provides essential support and a kind of suspension for the cellular organelles.

• It serves as a medium or a means for supplying organelles, it holds the organelles in the right position for their function and well-performing environment.

• Such interaction between the cytoplasm and cytoskeletal components creates an appropriate cell shape and prevents deformation that can arise under certain mechanical stresses.

• This interaction is extremely important for the spatial organisation of the cell and organelles within this cell.

Metabolic Activities

• Metabolic events in the cytoplasm are very important for cell survival and performance.

• The cytoplasm is a site for many biochemical events, also as glycolysis, leading to glucose processing and energy derivation.

• It is also one of the significant places in the biosynthesis of proteins. Ribosomes are located in the site and promote the translation of the information from mRNA into a peptide sequence.

• Cellular respiration in animal cells usually takes place in the mitochondria. Respiration starts in the cytoplasm, which therefore involves the cytoplasm in energy generation and the course of general metabolic reaction cycles.

Cellular Signalling

• Cytoplasm is essential for cellular signalling and molecular transport.

• It is used actively in intracellular transport, gaining movement of different molecular and cellular cargo necessary and sufficient for functional performance within a cell.

• This intracellular movement is mainly by molecular diffusion, but in many cases, it is realised by the movements of vesicles or other cellular fragments, transferred within the cytoplasm.

• Furthermore, cytoplasm has been known to be central to the signal transduction pathways that interface with the interactions at the plasma membrane by coupling these signalling events to the regulation of nuclear and organelle function.

• This is also essential for proper cellular physiology, as well as well-regulated cell-to-cell communication.

Dynamic Nature of the Cytoplasm

The cytoplasm is not a static fluid but a highly dynamic medium where continuous streaming and movement occur. This dynamic nature allows efficient transport of materials, proper distribution of organelles, and coordination of metabolic activities within the cell.

Cytoplasmic Streaming

• Cytoplasmic streaming, also known as cytoplasmic flow or protoplasmic streaming, is a process observed in many eukaryotic cells wherein the cytoplasm has constant, directed movement.

• The movement is significant for different cellular processes, wherein it is particularly common in large plant or fungal cells because it helps in the distribution of nutrients, organelles, and other cell components.

• This also enhances the efficient uptake of nutrients and excretion of waste within the cell, which also adds to the right concerns that the cell needs to sustain its metabolic processes.

• All these complement its functions towards promoting cell growth, development, and response to different environmental events.

Changes During Cell Division

• The dynamics of the cytoplasm are significantly different when the cell divides. This is more so within the cytokinesis, which marks the final step in cell division.

• In both the processes of mitosis and meiosis, cytokinesis is the step that delineates cytoplasmic differentiation.

• It is within this process that the cytoplasm is reorganised or redistributed. The reason for reorganisation is to ensure that each daughter cell has received the organelles and other cytoplasmic materials it needs in sufficient amounts to effectively survive and properly function.

• This reorganisation is a very crucial process that helps the cell maintain its integrity and continues the genetic information from the parent cell.

• The coordination between the dynamics of the cytoplasm and the cell cycle is very important for the distribution of cytoplasmic contents to be guaranteed and cell division to occur flawlessly, underscoring the dynamic nature of the cytoplasm in cellular approaches.

Differences Between Cytoplasm and Nucleus

Interaction between cytoplasm and nucleus:

• Organelle that acts as a brain for the cell, as it controls the gene expression and DNA age.

• The collaboration between the nucleus and the cytoplasm is extremely broad and sustains cellular activities.

• Signals in the cytoplasm may influence gene expression and the response of the cell through the signal transduction pathway.

• Many molecules—among them, both proteins and RNA molecules—pass back and forth between the cytoplasm and the nucleus for purposes of communication and transport of the genetic message.

Applications and Importance

The applications and importance of cytoplasm:

Medical Applications

Understanding cytoplasmic dysfunctions will help identify the mechanisms of pathogenesis and disease and lead to effective treatments. Specific targeting of cytoplasmic molecules will enable the development of innovative therapies against numerous pathological conditions.

Biotechnological Applications

The significance of cytoplasm in genetic engineering and cell research finds a foundation. The ability to control processes in the cytoplasm leads to possible changes in gene expression and the engineering of cellular functions, which stimulate the progress of biotechnology by making the development of new therapies and diagnostic tools easy.

Cytoplasm NEET MCQs (With Answers & Explanations)

Importance NEET topics are:

• Structure of Cytoplasm

• Functions of Cytoplasm

Practice Questions for NEET

Q1. A protoplast is a cell:

1. Without nucleus

2. Undergoing division

3. Without cell wall

4. Without plasma membrane

Correct answer: 3) Without cell wall

Explanation:

Protoplasts are cells with the plasma membrane, cytoplasm and nucleus, which have had their cell wall removed by the action of enzymes. Cellulase enzymes digest the cellulose in plant cell walls while pectinase enzymes break down the pectin holding cells together.

Hence, the correct answer is option 3) without a cell wall.

Q2. Non-living substances of the protoplasm are collectively known as

1. Deutoplasm

2. Plasmagel

3. Cytoplams

4. Mesoplasma

Correct answer: 1) Deutoplasm

Explanation:

The term "deutoplasm" refers to all of the non-living materials found in the protoplasm. These materials include the following and are frequently trash, secretion, or storage products:

• Food ingredients (such as starch in plant cells or glycogen in animal cells) should be kept in reserve.

• Droplets of fat

• Pigments

• Crystals like calcium oxalate

• Vacuoles that hold materials such as salts, water, or waste products from metabolism

Hence, the correct answer is option 1) Deutoplasm.

Q3. Given below are a few statements regarding somatic hybridization. Choose the correct statements.

(i) protoplasts of different cells of the same plant are fused

(ii) protoplasts from cells of different species can be fused

(iii) treatment of cells with cellulase and pectinase is mandatory

(iv) the hybrid protoplast contains characters of only one parental protoplast.

1. (i) and (iii)

2. (i) and (ii)

3. (i) and (iv)

4. (ii) and (iii)

Correct answer: (ii) and (iii)

Explanation:

The correct statements about somatic hybridization are:
(i) Protoplasts of different cells of the same plant are fused - This statement is wrong because somatic hybridization generally involves protoplasts from different species.
(ii) Protoplasts from cells of different species can be fused - This statement is correct. Somatic hybridization allows the fusion of protoplasts from different species, overcoming the barriers present in sexual reproduction.
(iii) Treatment of cells with cellulase and pectinase is indispensable - This is true. This step helps in the removal of the cell wall and protoplasts can then be isolated and fused.
(iv) The hybrid protoplast contains characteristics of only one parental protoplast - This statement is false. Generally, a hybrid protoplast is made up of the genetic material of both the parent protoplast and thus carries traits of the two. Hence, statements (ii) and (iii) are correct.

Hence, the correct answer is option 4) (ii) and (iii).

Also Read:

• MCQs on Cytoplasm

• Endomembrane System

• Nucleus