what will be there in practical for bsc microbiology

The semester wise distribution of the subject as well as practicals may differ from the university to university. I am providing you here the UGC guidelines for BSc Microbiology:

Bacteriology Practicals:

1. Preparation of different media: synthetic media BG-11, Complex media-Nutrient agar, McConkey agar, EMB agar. 2. Simple staining3. Negative staining 4. Gram’s staining 5. Acid fast staining-permanent slide only. 6. Capsule staining 7. Endospore staining. 8. Isolation of pure cultures of bacteria by streaking method. 9. Preservation of bacterial cultures by various techniques. 10. Estimation of CFU count by spread plate method/pour plate method. 11. Motility by hanging drop method.

Biochemistry practicals:

1. Properties of water, Concept of pH and buffers, preparation of buffers and Numerical problems to explain the concepts 2. Numerical problems on calculations of Standard Free Energy Change and Equilibrium constant 3. Standard Free Energy Change of coupled reactions 4. Qualitative/Quantitative tests for carbohydrates, reducing sugars, non reducing sugars 5. Qualitative/Quantitative tests for lipids and proteins 6. Study of protein secondary and tertiary structures with the help of models 7. Study of enzyme kinetics – calculation of Vmax , Km, Kcat values 8. Study effect of temperature, pH and Heavy metals on enzyme activity 9. Estimation of any one vitamin.

For Virology you will have:

1. Study of the structure of important animal viruses (rhabdo, influenza, paramyxo hepatitis B and retroviruses) using electron micrographs 2. Study of the structure of important plant viruses (caulimo, Gemini, tobacco ring spot, cucumber mosaic and alpha-alpha mosaic viruses) using electron micrographs 3. Study of the structure of important bacterial viruses (X 174, T4, λ) using electron micrograph. 4. Isolation and enumeration of bacteriophages (PFU) from water/sewage sample using double agar layer technique 5. Studying isolation and propagation of animal viruses by chick embryo technique 6. Study of cytopathic effects of viruses using photographs 7. Perform local lesion technique for assaying plant viruses.

For Microbial Physiology, the practical may be:

1. Study and plot the growth curve of E. coli by turbidometric and standard plate count methods. 2. Calculations of generation time and specific growth rate of bacteria from the graph plotted with the given data 3. Effect of temperature on growth of E. coli 4. Effect of pH on growth of E. coli 5. Effect of carbon and nitrogen sources on growth of E.coli 6. Effect of salt on growth of E. coli 7. Demonstration of alcoholic fermentation 8. Demonstration of the thermal death time and decimal reduction time of E. coli.

To know about the practicals related to the other papers of BSc Microbiology visit the link below:

https://www.ugc.ac.in/pdfnews/5495549_B.Sc.-Hons-Microbiology.pdf

I hope my answer helps. All the very best for your future endeavors!