how is insertional activation of an enzyme is used as selectable marker to d for the recombinant particles from the recombinant
Answer (1)
12th Feb, 2022
Hi reader ,
The insertion of rDNA into the coding sequence of an enzyme alpha-galactosidase leads to the inactivation of the enzyme called insertional inactivation. The recombinants do not produce a blue coloured colonies in the presence of chromogenic substrate while the non-recombinants produce a blue colour.
the recobinant give out white colour thus helping to differentiate between recombinants and non recombinants
i hope this helps
thank you
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