How will changing the ratio of ddNTPs to dNTPs impact the DNA sequencing procedure?

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How will changing the ratio of ddNTPs to dNTPs impact the DNA sequencing procedure?

lekhraj.kushwaha 10th Dec, 2022

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lekhraj.kushwaha 10th Dec, 2022

The distribution of DNA fragment lengths produced by Sanger sequencing depends on the ratio of dNTP to ddNTP being used.

The product will be shorter and closer to the primer if this ratio increases, whereas the product will be longer and farther from the primer if it decreases.

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