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What are stepwise procedure for analysis of chemical composition of biomolecules ?


Unknown Student 18th Mar, 2020
Answer (1)
Pratyay Islam 18th Mar, 2020

Hello Student ,

The composition of living tissue/biomolecules can be studied by chemical analysis. The stepwise procedure is as follows

  1. Living tissue is taken and grinded in trichloroacetic acid.
  2. Then the mixture is strained with a cheese cloth
  3. Filtrate and Retentate are obtained after straining.
  4. Filtrate contains the acid solube pool - Mol Wt of biomolecules- 18-1800 Daltons . These are biomicromolecules.
  5. Retentate contains Acid Insoluble pool - Mol Wt of biomolecules - >800 D. These are biomarcomolecules.

Note : Lipids are absent in biomicromolecular pool due to their non polar nature. They associate and form larger compounds.

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Comments (1)
18th Mar, 2020
Thanks a lot
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