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The absorption of aromatic amino acids ( phenyaniline, tyrosine, and tryptophan ) depend on their molecular Environment. Alterations in the solvent can be used to change the Environment of those aromatic side chains in a folded protein that exposed to the solvent.

Significance - The solvent perturbation technique of difference spectroscopy has been used to study the interaction of an antibody synthetic polypeptide antibody with it's homologous antigen. Antibody was obtained from a sheep which had been immunized with the random polymer .