Hello Kumar,

Microorganisms can be found in large quantities practically at any place on the planet, from the depths of the earth to the intestines of humans. These can only be seen under the magnification of a microscope since they are too small to be seen with the naked eye.

Most of the microorganisms have a diameter of 0.2 um and a length of 2-8 um, and come in a variety of shapes, from spheres to rods and spirals. They only seem coloured when they are part of a colony; lone will appear transparent. The bacterial cells will drift in and out of focus at high magnification, especially if the water layer between the cover glass and the slide is too thick.

Without the use of a microscope, microorganisms are too tiny to see. While some eucaryotes, such as protozoa, algae, and yeast, may be seen at magnifications of 200X-400X, most of the microorganisms require a magnification of 1000X. A 100X oil immersion objective and 10X eyepieces are required. They are difficult to spot even with a microscope unless they are coloured. The following steps will guide you through the process.

THank you!