Hii Riya,

No, you have to apply just once as the application is same just the location of the preference of your entrance test center is what makes it different wherein you'll select your preference and on the basis of availability you'll be given a slot. In the same form it asks you about the test center you prefer from the options available. generally the candidates who are not able to fill the form as soon as it get out get a test center in bangalore as it is the place where there final entrance test is taken and they don't have any  option available to choose from.

Hope it helps.

Thanks.