The use of RTS signals in computer networking allows devices to negotiate the use of the communication channel and helps to prevent collisions and improves the efficiency of data transfer. The RTS signal acts as a request for the channel, and the Clear to Send (CTS) signal from the receiving device acts as a confirmation that the channel is clear for use. This process helps to reduce the chances of data collisions and increases the efficiency of data transfer, allowing for faster and more reliable communication between devices.