Hello aspirant!

For Second round and Mop Up round of Deemed/ Central Universities, candidates are required to submit fresh choices.

So yes, you have to make fresh choices.
During the second round of online allotment process, the choice of higher preference will be considered for up-gradation for those candidates who give option to upgrade their choice at the time of admission at allotted Medical/Dental College. During Round-2, fresh allotment will be considered for those eligible candidates who could not be allotted seat due to non-availability of seat in Round-1, subject to availability of seat.

For more information refer to https://mcc.nic.in/MCCReg/Documents/PGFAQ18revisedlatest15-03-18.pdf