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Advanced Fluorescence Microscopy and Image Processing
Online
12 Weeks
Free Quick Facts
| Medium Of Instructions | Mode Of Learning | Mode Of Delivery |
|---|---|---|
| English | Self Study | Video and Text Based |
Courses and Certificate Fees
| Fees Informations | Certificate Availability | Certificate Providing Authority |
|---|---|---|
| INR 1000 | yes | IIT Hyderabad |
The Syllabus
- Introduction to fluorescence microscopy and its applications
- Introduction to Microscope Optics
- Design of a Fluorescence Microscope
- Fluorescent proteins, organic dyes, and protein labeling strategies
- 6D imaging, live cell imaging, time-lapse imaging, FRAP, FRET, FLIM
- Illumination strategies (Epi, TIRF, HILO, light sheet, multi-photon)
- Confocal Microscopy
- Super-resolution microscopy (SIM, STED, STORM/PALM)
- Live demonstration of a fluorescence microscope, 6D imaging of live cells
- Immunofluorescence (IF), Immunohistochemistry (IHC)
- Fluorescence in-situ Hybridization (FISH), RNAFISH
- Digital images and camera technologies for microscopy
- CCD, EMCCD, sCMOS camera
- ImageJ/FIJI-based image processing and data analysis (workshop, basic operations of ImageJ/FIJI)
- ImageJ/FIJI-based image processing and data analysis (workshop, image segmentation)
- ImageJ/FIJI-based image processing and data analysis (workshop, macros, intensity measurement, scale bar, time scale, montage preparation, colocalization)
- Single-molecule imaging and tracking
- Optical tweezers and traction force microscopy
- Spatial Transcriptomics and Proteomics (RNAscope, MERFISH, CODEX)
- High-content imaging
Designing fluorescence microscopy experiments
Instructors
Articles
3 Jun'22, 08:23 AM
3 Jun'22, 08:23 AM