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    Quick Facts

    Medium Of InstructionsMode Of LearningMode Of Delivery
    EnglishSelf StudyVideo and Text Based

    Courses and Certificate Fees

    Fees InformationsCertificate AvailabilityCertificate Providing Authority
    INR 1000yesIIT Kharagpur

    The Syllabus

    • Basic Concepts including Central Dogma in Molecular Biology Definition and Scope of Molecular Diagnostics and Historical Developments Importance and advantages of molecular diagnostics over traditional methods
    • Nucleic Acid Structure and Function
    • DNA Replication and Repair
    • RNA Transcription and Processing 
    • Protein Synthesis from mRNA-Translation

    • Tools of Molecular diagnostics and Gene expression Analysis (I) PCR (Polymerase Chain Reaction) Fundamentals
    • RT PCR and qPCR
    • Modifications of PCR-Hot start
    • Touch down, nested PCR
    • Multiplex, Modifications of PCR 2-Long-range PCR,Single-cell PCR,Fast-cycling PCR 
    • Methylation-specific PCR (MSP)
    • Digital Droplet PCR-modern implications, PCR-based mutation analysis

    • Tools of Molecular diagnostics and Gene expression Analysis (II) Principles (Sanger sequencing, Overview of NGS Technologies and Platform, 
    • Application of NGS in Molecular Diagnostics
    • Clinical Interpretation of NGS Data
    • Whole genome vs Whole exome sequencing 
    • Targeted gene panels, NGS library preparations)

    • Tools of Molecular diagnostics and Gene expression Analysis (III) DNA Microarray
    • FISH (Fluorescence in situ Hybridization)
    • Serial analysis of gene expression 
    • RNA sequencing
    • Tiling array
    • DNA protein interaction- chromatin immune precipitation

    • Techniques of Gene Manipulation
    • RNA interference and detection methods
    • Recombinant DNA Technology
    • CrispR-CAS9 technology
    • Epigenetics and diseases
    • DNA methylation analysis

    • Proteomics: Clinical Applications Overview of proteomics techniques and workflows, 
    • Protein separation techniques-brief discussion of gel electrophoresis and chromatography, mass spectrometry, label-free and isotope labelling methods, role of metabolomics in laboratory diagnosis

    • Proteomics: Advanced topics in Clinical Proteomics High throughput proteomics like-Shotgun and data independent acquisition (DIA)
    • Single cell proteomics and spatial profiling, methods to detect post translational modification and protein-protein interaction, proteomic data analysis and bioinformatic tools Luminex multiplex assays and its application in biomarker analysis

    • Molecular Diagnostics in Infectious Diseases Syndromic Panels and Multiplex Assay, Molecular identification of Microorganism- covering bacterial, viral, fungal and parasitic diseases, antimicrobial resistance testing
    • POC Molecular diagnostics for infectious diseases
    • Molecular diagnostics in Hospital acquired infections.

    • Molecular Diagnostics in Cancer Management Cancer markers
    • Liquid biopsies in cancer detection, circulating Tumour DNA (ctDNA) analysis 
    • Monitoring treatment response with molecular diagnostics 
    • Molecular diagnostics in targeted therapy
    • Digital PCR, Molecular diagnostics quality control

    • Molecular Diagnostics in Genetic and Inherited Disorders Genetic testing and inherited diseases 
    • Non-Invasive Prenatal testing (NIPT) and reproductive genetics
    • Molecular diagnostics in rare genetic disorders 
    • Pharmacogenomics and Personalized Medicine, genetic counselling and patient education

    • Molecular Diagnostics in Medicine Molecular diagnostics in Metabolic disease
    • Molecular diagnostics in Neurodegenerative disease
    • Molecular diagnostics in Respiratory
    • Molecular diagnostics in Gastrointestinal disorders
    • Molecular diagnostics in Endocrine disorders 
    • Molecular diagnostics in Autoimmune disorders
    • Molecular diagnostics in Cardiovascular diseases
    • Molecular diagnostics in Transplantation diseases

    • Molecular Diagnostics: Quality control and Ethical Concerns in and Futuristic Trends Quality control in molecular diagnostics
    • Ethical Concerns in Molecular Diagnostics
    • Microfluidics and Lab-on-chip in molecular diagnostics 
    • AI and ML in molecular diagnostics 
    • Nanotechnology based molecular diagnostics 
    • Single cell Analysis
    • Integration of Multi-omics Data

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