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Quick Facts

Medium Of InstructionsMode Of LearningMode Of Delivery
EnglishSelf StudyVideo and Text Based

Courses and Certificate Fees

Fees InformationsCertificate AvailabilityCertificate Providing Authority
INR 1000yesIIT Kharagpur

The Syllabus

  • Basic Concepts including Central Dogma in Molecular Biology Definition and Scope of Molecular Diagnostics and Historical Developments Importance and advantages of molecular diagnostics over traditional methods
  • Nucleic Acid Structure and Function
  • DNA Replication and Repair
  • RNA Transcription and Processing 
  • Protein Synthesis from mRNA-Translation

  • Tools of Molecular diagnostics and Gene expression Analysis (I) PCR (Polymerase Chain Reaction) Fundamentals
  • RT PCR and qPCR
  • Modifications of PCR-Hot start
  • Touch down, nested PCR
  • Multiplex, Modifications of PCR 2-Long-range PCR,Single-cell PCR,Fast-cycling PCR 
  • Methylation-specific PCR (MSP)
  • Digital Droplet PCR-modern implications, PCR-based mutation analysis

  • Tools of Molecular diagnostics and Gene expression Analysis (II) Principles (Sanger sequencing, Overview of NGS Technologies and Platform, 
  • Application of NGS in Molecular Diagnostics
  • Clinical Interpretation of NGS Data
  • Whole genome vs Whole exome sequencing 
  • Targeted gene panels, NGS library preparations)

  • Tools of Molecular diagnostics and Gene expression Analysis (III) DNA Microarray
  • FISH (Fluorescence in situ Hybridization)
  • Serial analysis of gene expression 
  • RNA sequencing
  • Tiling array
  • DNA protein interaction- chromatin immune precipitation

  • Techniques of Gene Manipulation
  • RNA interference and detection methods
  • Recombinant DNA Technology
  • CrispR-CAS9 technology
  • Epigenetics and diseases
  • DNA methylation analysis

  • Proteomics: Clinical Applications Overview of proteomics techniques and workflows, 
  • Protein separation techniques-brief discussion of gel electrophoresis and chromatography, mass spectrometry, label-free and isotope labelling methods, role of metabolomics in laboratory diagnosis

  • Proteomics: Advanced topics in Clinical Proteomics High throughput proteomics like-Shotgun and data independent acquisition (DIA)
  • Single cell proteomics and spatial profiling, methods to detect post translational modification and protein-protein interaction, proteomic data analysis and bioinformatic tools Luminex multiplex assays and its application in biomarker analysis

  • Molecular Diagnostics in Infectious Diseases Syndromic Panels and Multiplex Assay, Molecular identification of Microorganism- covering bacterial, viral, fungal and parasitic diseases, antimicrobial resistance testing
  • POC Molecular diagnostics for infectious diseases
  • Molecular diagnostics in Hospital acquired infections.

  • Molecular Diagnostics in Cancer Management Cancer markers
  • Liquid biopsies in cancer detection, circulating Tumour DNA (ctDNA) analysis 
  • Monitoring treatment response with molecular diagnostics 
  • Molecular diagnostics in targeted therapy
  • Digital PCR, Molecular diagnostics quality control

  • Molecular Diagnostics in Genetic and Inherited Disorders Genetic testing and inherited diseases 
  • Non-Invasive Prenatal testing (NIPT) and reproductive genetics
  • Molecular diagnostics in rare genetic disorders 
  • Pharmacogenomics and Personalized Medicine, genetic counselling and patient education

  • Molecular Diagnostics in Medicine Molecular diagnostics in Metabolic disease
  • Molecular diagnostics in Neurodegenerative disease
  • Molecular diagnostics in Respiratory
  • Molecular diagnostics in Gastrointestinal disorders
  • Molecular diagnostics in Endocrine disorders 
  • Molecular diagnostics in Autoimmune disorders
  • Molecular diagnostics in Cardiovascular diseases
  • Molecular diagnostics in Transplantation diseases

  • Molecular Diagnostics: Quality control and Ethical Concerns in and Futuristic Trends Quality control in molecular diagnostics
  • Ethical Concerns in Molecular Diagnostics
  • Microfluidics and Lab-on-chip in molecular diagnostics 
  • AI and ML in molecular diagnostics 
  • Nanotechnology based molecular diagnostics 
  • Single cell Analysis
  • Integration of Multi-omics Data

Instructors

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