Quick Facts

Medium Of Instructions	Mode Of Learning	Mode Of Delivery
English	Self Study	Video and Text Based

Important dates

Start Date : 25 Apr, 2026

Fees Informations	Certificate Availability	Certificate Providing Authority
INR 1000	yes	IIT Kharagpur

Basic Concepts including Central Dogma in Molecular Biology Definition and Scope of Molecular Diagnostics and Historical Developments Importance and advantages of molecular diagnostics over traditional methods
Nucleic Acid Structure and Function
DNA Replication and Repair
RNA Transcription and Processing
Protein Synthesis from mRNA-Translation

Tools of Molecular diagnostics and Gene expression Analysis (I) PCR (Polymerase Chain Reaction) Fundamentals
RT PCR and qPCR
Modifications of PCR-Hot start
Touch down, nested PCR
Multiplex, Modifications of PCR 2-Long-range PCR,Single-cell PCR,Fast-cycling PCR
Methylation-specific PCR (MSP)
Digital Droplet PCR-modern implications, PCR-based mutation analysis

Tools of Molecular diagnostics and Gene expression Analysis (II) Principles (Sanger sequencing, Overview of NGS Technologies and Platform,
Application of NGS in Molecular Diagnostics
Clinical Interpretation of NGS Data
Whole genome vs Whole exome sequencing
Targeted gene panels, NGS library preparations)

Tools of Molecular diagnostics and Gene expression Analysis (III) DNA Microarray
FISH (Fluorescence in situ Hybridization)
Serial analysis of gene expression
RNA sequencing
Tiling array
DNA protein interaction- chromatin immune precipitation

Proteomics: Clinical Applications Overview of proteomics techniques and workflows,
Protein separation techniques-brief discussion of gel electrophoresis and chromatography, mass spectrometry, label-free and isotope labelling methods, role of metabolomics in laboratory diagnosis

Proteomics: Advanced topics in Clinical Proteomics High throughput proteomics like-Shotgun and data independent acquisition (DIA)
Single cell proteomics and spatial profiling, methods to detect post translational modification and protein-protein interaction, proteomic data analysis and bioinformatic tools Luminex multiplex assays and its application in biomarker analysis

Molecular Diagnostics in Infectious Diseases Syndromic Panels and Multiplex Assay, Molecular identification of Microorganism- covering bacterial, viral, fungal and parasitic diseases, antimicrobial resistance testing
POC Molecular diagnostics for infectious diseases
Molecular diagnostics in Hospital acquired infections.

Molecular Diagnostics in Genetic and Inherited Disorders Genetic testing and inherited diseases
Non-Invasive Prenatal testing (NIPT) and reproductive genetics
Molecular diagnostics in rare genetic disorders
Pharmacogenomics and Personalized Medicine, genetic counselling and patient education

Molecular Diagnostics: Quality control and Ethical Concerns in and Futuristic Trends Quality control in molecular diagnostics
Ethical Concerns in Molecular Diagnostics
Microfluidics and Lab-on-chip in molecular diagnostics
AI and ML in molecular diagnostics
Nanotechnology based molecular diagnostics
Single cell Analysis
Integration of Multi-omics Data