The inauguration followed by an
overview of all day’s hands-on
training events information in
detail. Pre-requisites helpful
information for all hands-on
training experiments.
RNA isolation from DNA/RNA virusinfected cells (Trizol method).
Visualization, quantitation, and
analysis of RNA from virus-infected
host cells (RNA gel electrophoresis,
nanodrop, spectroscopy)
RNA to cDNA conversion and
preparation of real-time PCR
plate (PCR, RT-PCR)
Real-time PCR experiments with
various primers and analysis the
results (qRT-PCR)
Virus-infected cells biomolecular
spectrum study compared to
controls (Raman
microspectroscopy)
Analysis of biomolecular signature
obtained from Raman spectroscopy
(Analysis and result interpretation,
Principal component analysis)
Protein biomarkers study
through Immunofluorescence
study (Immuno-florescence
methods)
Protein biomarkers observation
through a Fluorescence microscope
along with nuclear staining
(Fluorescence microscope)
Preparation of virus-infected
cells for cell sorting and
Immunophenotyping (Flow
cytometer protocol)
Using cell sorting &
Immunophenotyping of Virus
infected cells (BD FACSARIA III &
(BD FACSARIA III)
Large scale Machine learning
algorithm& applications to
Biomedical Data
Deep Learning approaches for
Biomedical data
Overall assessment, test, and
feedback session
Valedictory function and certificate
distribution to participants,
volunteers, and faculties.