Biotechnology MCQs: Objective Questions with Answers

MCQs

1. The discovery of restriction enzymes was made by

1. Smith and Nathans

2. Alexander Fleming

3. Berg

4. None

2. By slicing apart viral DNA, bacteria defend themselves from viruses.

1. Ligase

2. Endonuclease

3. Exonuclease

4. Gyrase

3. The Klenow fragment comes from

1. DNA Ligase

2. DNA Pol-I

3. DNA Pol-II

4. Reverse Transcriptase

4. ELISA test is

1. Using a second radiolabelled antibody

2. The use of RBCs

3. Using cell lysis caused by complement

4. Adding a material that transforms into a coloured final product

5. The Golden Rice variety contains a lot of

1. Vitamin C

2. Β-carotene and ferritin

3. Biotin

4. Lysine

6. The sticky ends of the DNA fragments are the result of

1. Endonuclease

2. Unpaired bases

3. Calcium ions

4. Free methylation

7. Which of the following justifies the use of plasmids as cloning vectors?

1. In culture, they can be multiplied

2. Bacterial cells that replicate themselves

3. Can be multiplied using enzymes in a laboratory

4. Freely replicate outside of bacterial cells

8. The year saw the beginning of the human genome project.

1. 1980

2. 1973

3. 1990

4. 1989

9. The vaccines prepared through recombinant DNA technology are

1. Third generation vaccines

2. First-generation vaccines

3. Second-generation vaccines

4. None

10. Which crop has undergone genetic modification?

1. Bt-cotton

2. Bt-brinjal

3. Golden rice

4. All

Also read

• Biotechnology: Principles And Processes

• Restriction Enzyme

• Cloning Vector

• Biotechnology And Its Applications

11. The PCR method was created by

1. Karry Mullis

2. Boyer

3. Sanger

4. Cohn

12. The initial transgenic plant that has been created is

1. Brinjal

2. Tobacco

3. Rice

4. Cotton

13. RNA interference facilitates

1. Cell proliferation

2. Micropropagation

3. Cell defense

4. Cell differentiation

14. Which of the following describes the enhanced transgenic basmati rice's quality?

1. A high yield is produced, but no distinctive aroma.

2. High output and high vitamin A content

3. Does not require growth hormones or chemical fertilisers

4. Able to fend off illnesses and insects

15. The first clinical use of gene therapy included a 4-year-old girl, for

1. Adenosine deaminase deficiency

2. Adenosine deficiency

3. Growth deficiency

4. Adenine deficiency

16. A gene's insertion and deletion are referred to as

1. Biotechnology

2. Genetic engineering

3. Cytogenetics

4. Gene therapy

17. A transgene's expression in the target tissue is detected using a

1. Transgene

2. Promoter

3. Enhancer

4. Reporter

18. ——- is a vector that is utilised to clone into higher organisms.

1. Retrovirus

2. Baculovirus

3. Salmonella typhimurium

4. Rhizopus nigricans

19. Which bacterium is used in the production of insulin by genetic engineering?

1. Saccharomyces

2. Rhizobium

3. Escherichia

4. Mycobacterium

20. Who Coined the Phrase "Biotechnology"?

1. National Center for Biotechnology Information (NCBI)

2. The European Federation of Biotechnology (EFB)

3. National Centre for Cell Science (NCCS)

4. National Institutes of Health (NIH)

21. Mention the two points of view that the term "biotechnology" encompasses.

1. Traditional biotechnology and Modern molecular biotechnology

2. Medical biotechnology and Agricultural biotechnology

3. Green biotechnology and Animal biotechnology

4. Blue biotechnology and Red biotechnology

22. The first recombinant DNA was produced using the bacterium ____'s plasmid.

1. Bacillus subtilis

2. Cyanobacteria

3. Salmonella typhimurium

4. Saccharomyces cerevisiae

23. The act of producing several copies of the desired DNA template is referred to as

1. Transferring

2. Cloning

3. Genetic engineering

4. R-DNA technology

24. The drawbacks of conventional hybridisation methods are overcome by _______ approaches.

1. Modern Hybridization

2. Immunology

3. Cell Biology

4. Genetic engineering

25. What are the two main methods that made contemporary biotechnology possible?

1. Classical and traditional biotechnology

2. Red biotechnology and green biotechnology

3. Genetic engineering and maintenance of a sterile environment

4. Genetics and mathematics

Answer Key

1. Smith and Nathans

2. Endonuclease

3. DNA Pol-I

4. Adding a material that transforms into a coloured final product

5. Β-carotene and ferritin

6. Unpaired bases

7. In culture, they can be multiplied

8. 1990

9. Third generation vaccines

10. All

11. Karry Mullis

12. Tobacco

13. Cell defense

14. High output and high vitamin A content

15. Adenosine deaminase deficiency

16. Genetic engineering

17. Reporter

18. Retrovirus

19. Escherichia

20. The European Federation of Biotechnology (EFB)

21. Traditional biotechnology and Modern molecular biotechnology

22. Salmonella typhimurium

23. Cloning

24. Genetic engineering

25. Genetic engineering and maintenance of a sterile environment

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